LOL, no... one key file for each softcam - i.e. if you just use s2emu, then you just need one version of softcam.key.
If you use Krasnal for example, then you need a different named file (keys.bin), and not softcam.key.
Generally speaking, each line in the file supplies one key, so make sure you have the latest file if it appears that some channels don't work.
Many files still contain keys for channels that are no longer available (even if we have the correct 'new' keys) due to other code changes (D+ for example).
You
can edit it (and it's a good introduction into how it all works) but just download the latest one if you can't be arsed!
Make a file backup before you start.
STICK